Learn how to implement strategies to achieve satisfactory separation for 'complex' samples and use computer modelling to develop robust and fit for purpose HPLC methods.
This course is ideal for those who have experience of developing HPLC methods but want to increase their knowledge to deal with more challenging separations.
Course Outline
鈥� Reasons why some separations are 'challenging'
鈥� Samples containing large numbers of analytes and/or complex matrix
鈥� Problematic molecules and mixtures
鈥� Implementing a 5-step strategy for method development of complex samples (see 'How to Develop HPLC Methods')
鈥� Using computer simulation to develop fit for purpose HPLC methods
This course focuses on reversed phase mode separations.
This course is ideal for those who have experience of developing HPLC methods but want to increase their knowledge to deal with more challenging separations.
Course Outline
鈥� Reasons why some separations are 'challenging'
鈥� Samples containing large numbers of analytes and/or complex matrix
鈥� Problematic molecules and mixtures
鈥� Implementing a 5-step strategy for method development of complex samples (see 'How to Develop HPLC Methods')
鈥� Using computer simulation to develop fit for purpose HPLC methods
This course focuses on reversed phase mode separations.
